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Anti-BrdU Antibody [YR-BrdU] (A58171)

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$415

Mouse monoclonal [YR-BrdU] antibody to BrdU for IHC and Flow Cytometry.

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Name
Anti-BrdU Antibody [YR-BrdU]
Description
Mouse monoclonal [YR-BrdU] antibody to BrdU.
Applications
IHC, Flow Cytometry
Dilutions
Dependending on Assay: 1:500-1:2,000.
Immunogen
5-iodouridine covalently coupled to KLH.
Host
Mouse
Clonality
Monoclonal
Clone ID
YR-BrdU
Isotype
IgG1
Conjugate

Unconjugated

Purification
Protein A chromatography.
Concentration
1mg/ml
Product Form
Lyophilized
Reconstitution
Reconstitute with sterile water. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Formulation
Lyophilized from Phosphate Buffered Saline.
Storage
Shipped at 4°C. Lyophilized: Long term store at 2-8°C in a dry environment. Reconstituted: Aliquot and store at -20°C long term or use within a month at 4°C. Avoid freeze/thaw cycles.
General Notes
This antibody was produced in BALB/c mice. This antibody can be used to detect proliferating cells in living tissues after incubation with BrdU which will be incorporated into newly synthesized DNA of replicating cells (during the S phase of the cell cycle), in place of thymidine during DNA replication. Antibody binding requires prior denaturation of the DNA, by exposing the cells to acid or heat.
Synonyms
Bromodeoxyuridine, BUdr
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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