Anti-Brd4 (phospho Thr204) Antibody [ARC50960] (A305446)

Name: Anti-Brd4 (phospho Thr204) Antibody [ARC50960]
Brand: Antibodies.com
SKU: A305446
Price: 540.0 USD
Availability: InStock

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$540

Rabbit monoclonal [ARC50960] antibody to Brd4 (phospho Thr204) for WB.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Brd4 (phospho Thr204) Antibody [ARC50960]

Description

Rabbit monoclonal [ARC50960] antibody to Brd4 (phospho Thr204).

Applications

Dilutions

WB: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around T204 of human BRD4 (NP_490597.1).

Sequence

ASTPP

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC50960

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

200 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BRD4-NUT FUSION, BRD4-NUT fusion oncoprotein, BRD4_HUMAN, Bromodomain containing 4, bromodomain containing protein 4, Bromodomain-containing protein 4, CAP, chromosome associated protein, HUNK1, HUNKI, MCAP, Mitotic chromosome-associated protein, Protein HUNK1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-Brd4 (phospho Thr204) Antibody [ARC50960] (A305446)

Western blot analysis of extracts of various cell lines, using Anti-Brd4 (phospho Thr204) Antibody [ARC50960] (A305446) at 1:1,000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. C6 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-Brd4 (phospho Thr204) Antibody [ARC50960] (A305446)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-Brd4 (phospho Thr204) Antibody [ARC50960] (A305446) at 1:1,000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) for 30 minutes with NaCl (400mM) after serum-starvation 16-20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

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