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Anti-Brd4 (phospho Thr204) Antibody (A305447)

Western Blot - Anti-Brd4 (phospho Thr204) Antibody (A305447) - Antibodies.com
$435
Product Datasheet

Rabbit polyclonal antibody to Brd4 (phospho Thr204) for WB.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Brd4 (phospho Thr204) Antibody
Description
Rabbit polyclonal antibody to Brd4 (phospho Thr204).
Applications
WB
Dilutions
WB: 1:500-1:1,000
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphorylated peptide around T204 of human BRD4 (NP_490597.1).
Sequence
ASTPP
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
200 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
BRD4-NUT FUSION, BRD4-NUT fusion oncoprotein, BRD4_HUMAN, Bromodomain containing 4, bromodomain containing protein 4, Bromodomain-containing protein 4, CAP, chromosome associated protein, HUNK1, HUNKI, MCAP, Mitotic chromosome-associated protein, Protein HUNK1
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Brd4 (phospho Thr204) Antibody (A305447) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Brd4 (phospho Thr204) Antibody (A305447)
Western blot analysis of extracts of NIH/3T3 cells, using Anti-Brd4 (phospho Thr204) Antibody (A305447) at 1:1,000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
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