Anti-Brd4 Antibody [ARC0699] (A306296)

Name: Anti-Brd4 Antibody [ARC0699]
Brand: Antibodies.com
SKU: A306296
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0699] antibody to Brd4 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Brd4 Antibody [ARC0699]

Description

Rabbit monoclonal [ARC0699] antibody to Brd4.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human BRD4 (O60885).

Sequence

IIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGTAKPGVSTVPNTT

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0699

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

120 kDa / 140 kDa / 200 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BRD4-NUT FUSION, BRD4-NUT fusion oncoprotein, BRD4_HUMAN, Bromodomain containing 4, bromodomain containing protein 4, Bromodomain-containing protein 4, CAP, chromosome associated protein, HUNK1, HUNKI, MCAP, Mitotic chromosome-associated protein, Protein HUNK1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-Brd4 Antibody [ARC0699] (A306296)

Western blot analysis of extracts of various cell lines, using Anti-Brd4 Antibody [ARC0699] (A306296) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-Brd4 Antibody [ARC0699] (A306296)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Brd4 Antibody [ARC0699] (A306296) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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