Anti-BRD2 Antibody (A87828)

Name: Anti-BRD2 Antibody
Brand: Antibodies.com
SKU: A87828

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This product is discontinued

Anti-BRD2 Antibody (A87828) has been discontinued and is no longer available.

View all BRD2 Antibodies.

Specifications

Name

Anti-BRD2 Antibody

Description

Rabbit polyclonal antibody to BRD2.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human BRD2 (NP_005095.1).

Sequence

LLKSLHSAGPPLLAVTAAPPAQPLAKKKGVKRKADTTTPTPTAILAPGSPASPPGSLEPKAARLPPMRRESGRPIKPPRKDLPDSQQQHQSSKKGKLSEQL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

110 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Bromodomain-containing protein 2, KIAA9001, O27.1.1, RING3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-BRD2 Antibody (A87828)

Western blot analysis of extracts of mouse brian, using Anti-BRD2 Antibody (A87828) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Western Blot - Anti-BRD2 Antibody (A87828)

Western blot analysis of extracts of HeLa cells, using Anti-BRD2 Antibody (A87828) at 1:1,000 dilution. HeLa cells were treated by NaCl (400mM)The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunohistochemistry - Anti-BRD2 Antibody (A87828)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-BRD2 Antibody (A87828) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-BRD2 Antibody (A87828)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-BRD2 Antibody (A87828) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.