This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene.

Applications

ELISA

Dilutions

ELISA: 2-4 µg/ml

Reactivity

Human

Immunogen

Recombinant fragment, around amino acids 445-620, of human BRCA1 protein. The exact sequence is proprietary.

Host

Mouse

Clonality

Monoclonal

Clone ID

BRCA1/2986

Isotype

IgG1

Light Chains

kappa

Conjugate

Unconjugated

Purification

Protein A/G chromatography.

Concentration

200 µg/ml

Molecular Weight

220 kDa

Product Form

Liquid

Formulation

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Notes

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-BRCA1 Antibody [BRCA1/2986] - BSA and Azide free (A253237).

Synonyms

BRCA 1, BRCA1 DNA repair associated, BRCA1/BRCA2 containing complex subunit 1, BRCA1/BRCA2-containing complex, subunit 1, BRCA1_HUMAN, BRCAI, BRCC 1, BRCC1, Breast and ovarian cancer susceptibility protein 1, Breast Cancer 1, Breast Cancer 1 Early Onset, Breast cancer type 1 susceptibility protein, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, Protein phosphatase 1 regulatory subunit 53, PSCP, RING finger protein 53, RNF53

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (1)

Enlarge Image

Figure 1: Anti-BRCA1 Antibody [BRCA1/2986] (A250057)

Analysis of protein array containing more than 19,000 full-length human proteins using Anti-BRCA1 Antibody [BRCA1/2986]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt^TM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt^TM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

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