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Anti-BRCA1 Antibody (A80775)

Western Blot - Anti-BRCA1 Antibody (A80775) - Antibodies.com Western Blot - Anti-BRCA1 Antibody (A80775) - Antibodies.com Immunofluorescence - Anti-BRCA1 Antibody (A80775) - Antibodies.com Immunofluorescence - Anti-BRCA1 Antibody (A80775) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to BRCA1 for WB and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-BRCA1 Antibody
Description
Rabbit polyclonal antibody to BRCA1.
Applications
WB, ICC/IF
Dilutions
WB: 1:100-1:500, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 400-699 of human BRCA1 (NP_009230.2).
Sequence
LDDRWYMHSCSGSLQNRNYPSQEELIKVVDVEEQQLEESGPHDLTETSYLPRQDLEGTPYLESGISLFSDDPESDPSEDRAPESARVGNIPSSTSALKVPQLKVAESAQSPAAAHTTDTAGYNAMEESVSREKPELTASTERVNKRMSMVVSGLTPEEFMLVYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRARESQDRKIFRGLEICCYGPFTNMPTGCPPNCGCAARCLDRGQWLPCNWADV
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
BRCA 1, BRCA1 DNA repair associated, BRCA1/BRCA2 containing complex subunit 1, BRCA1/BRCA2-containing complex, subunit 1, BRCA1_HUMAN, BRCAI, BRCC 1, BRCC1, Breast and ovarian cancer susceptibility protein 1, Breast Cancer 1, Breast Cancer 1 Early Onset, Breast cancer type 1 susceptibility protein, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, Protein phosphatase 1 regulatory subunit 53, PSCP, RING finger protein 53, RNF53
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-BRCA1 Antibody (A80775) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-BRCA1 Antibody (A80775)
Western blot analysis of extracts of various cell lines, using Anti-BRCA1 Antibody (A80775) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.
Western Blot - Anti-BRCA1 Antibody (A80775) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-BRCA1 Antibody (A80775)
Western blot analysis of extracts of various cell lines, using Anti-BRCA1 Antibody (A80775) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunofluorescence - Anti-BRCA1 Antibody (A80775) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-BRCA1 Antibody (A80775)
Immunofluorescence analysis of C6 cells using Anti-BRCA1 Antibody (A80775) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-BRCA1 Antibody (A80775) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-BRCA1 Antibody (A80775)
Immunofluorescence analysis of U-2OS cells using Anti-BRCA1 Antibody (A80775) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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