Anti-BNP Antibody (A11515)

Name: Anti-BNP Antibody
Brand: Antibodies.com
SKU: A11515
Price: 335.0 USD
Availability: InStock

Enlarge Image

$335

Rabbit polyclonal antibody to BNP for WB, IHC and ICC/IF.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-BNP Antibody

Description

Rabbit polyclonal antibody to BNP.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-134 of human BNP (NP_002512.1).

Sequence

MDPQTAPSRALLLLLFLHLAFLGGRSHPLGSPGSASDLETSGLQEQRNHLQGKLSELQVEQTSLEPLQESPRPTGVWKSREVATEGIRGHRKMVLYTLRAPRSPKMVQGSGCFGRKMDRISSSSGLGCKVLRRH

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

15 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BNP(1-28), BNP(1-29), BNP(1-30), BNP(1-32), BNP(2-31), BNP(3-29), BNP(3-30), BNP(3-32), BNP(4-27), BNP(4-29), BNP(4-30), BNP(4-31), BNP(4-32), BNP(5-29), BNP(5-31), BNP(5-32), Brain natriuretic peptide 32, Brain type natriuretic peptide, Gamma brain natriuretic peptide, Natriuretic peptide B, Natriuretic peptide precursor B, natriuretic peptide, brain type, Natriuretic peptides B, natriuretic protein, NPPB

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

Enlarge Image

Figure 1: Western Blot - Anti-BNP Antibody (A11515)

Western blot analysis of Mouse brain, using Anti-BNP Antibody (A11515) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Enlarge Image

Figure 2: Immunohistochemistry - Anti-BNP Antibody (A11515)

Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-BNP Antibody (A11515) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-BNP Antibody (A11515)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-BNP Antibody (A11515) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunofluorescence - Anti-BNP Antibody (A11515)

Immunofluorescence analysis of rat heart cells using Anti-BNP Antibody (A11515) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.