Anti-BMP4 Antibody [ARC0587] (A308533)

Western blot analysis of extracts of various cell lines, using Anti-BMP4 Antibody [ARC0587] (A308533) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-BMP4 Antibody [ARC0587] (A308533) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Confocal imaging of HeLa cells using Anti-BMP4 Antibody [ARC0587] (A308533), at a dilution of 1:50, (red). The cells were counterstained with Anti-alpha Tubulin Antibody, at a dilution of 1:400, (green). DAPI was used for nuclear staining (Blue). Objective: 60x.