Anti-Bmi1 Antibody (A12543)

Name: Anti-Bmi1 Antibody
Brand: Antibodies.com
SKU: A12543
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Bmi1 for WB and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Bmi1 Antibody

Description

Rabbit polyclonal antibody to Bmi1.

Applications

WB, IP

Dilutions

WB: 1:500-1:2,000, IP: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Bmi1 (NP_005171.4).

Sequence

LKDYYTLMDIAYIYTWRRNGPLPLKYRVRPTCKRMKISHQRDGLTNAGELESDSGSDKANSPAGGIPSTSSCLPSPSTPVQSPHPQFPHISSTMNGTSNSP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

43 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

B lymphoma Mo MLV insertion region (mouse), B lymphoma Mo MLV insertion region 1 homolog, Bmi 1, BMI1 polycomb ring finger oncogene, BMI1_HUMAN, Flvi 2/bmi 1, FLVI2/BMI1, MGC12685, Murine leukemia viral (bmi 1) oncogene homolog, Oncogene BMI 1, PCGF 4, PCGF4, Polycomb complex protein BMI 1, Polycomb complex protein BMI-1, Polycomb group protein Bmi1, Polycomb group ring finger 4, Polycomb group RING finger protein 4, RING finger protein 51, RNF 51, RNF51

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Bmi1 Antibody (A12543)

Western blot analysis of extracts of various cell lines, using Anti-Bmi1 Antibody (A12543) at 1:5,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-Bmi1 Antibody (A12543)

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Figure 3: Western Blot - Anti-Bmi1 Antibody (A12543)

Immunoprecipitation analysis of 200µg extracts of K-562 cells using 3µg of Anti-Bmi1 Antibody (A12543). This Western blot was performed on the immunoprecipitate using Anti-Bmi1 Antibody (A12543) at a dilution of 1:1000.

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