BLUelf Prestained Protein Ladder (A318980)

BLUelf Prestained Protein Ladder

Pre-stained protein ladder. Covers the size range 3.5-245 kDa (Tris-glycine-SDS running buffer). Number of bands = 13. Green band at approximately 25 kDa. Red band at approximately 75 kDa. Designed for monitoring SDS-PAGE separation and WB transfer efficiency. Thaw at room temperature or 37-40°C for a few minutes prior to use to dissolve precipitated solids. Do not boil. Do not heat, dilute, or add reducing agent before loading. Mix thoroughly.

SDS-PAGE, WB

0.1-0.4 mg/ml of each protein

Liquid

20 mM Tris-Phosphate pH7.5, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea and 15% (v/v) Glycerol.

Shipped at 4°C. Store at 25°C for up to 2 weeks, 4°C for up to 3 months, and -20°C for long-term storage.

This product is for research use only. It is not intended for diagnostic or therapeutic use.