Mouse monoclonal [HE-195] antibody to Blood Group A Antigen.
Specificity
This antibody recognizes human blood group A including weak variants A3, AX, A3B, AXB. The specifity of this antibody was confirmed by comparison of specifity and reactivity to standard reagent using >5.000 samples of blood.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1-4 µg/ml.
Reactivity
Human
Immunogen
Mixture of erythrocytes of group A1 and glycoprotein fraction isolated from saliva of secretors with blood group A.
Host
Mouse
Clonality
Monoclonal
Clone ID
HE-195
Isotype
IgM
Conjugate
Unconjugated
Purification
Purified by differential precipitation and solid-phase chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 8, with 15 mM Sodium Azide.
Figure 1: Flow Cytometry - Anti-Blood Group A Antigen Antibody [HE-195] (A254421)
Flow cytometric analysis of human peripheral whole blood stained with Anti-Blood Group A Antibody [HE-195] (concentration in sample 3.3µg/ml, GAM-APC).
Figure 2: Flow Cytometry - Anti-Blood Group A Antigen Antibody [HE-195] (A254421)
Separation of erythrocytes stained with Anti-Blood Group A Antibody [HE-195] (concentration in sample 3.3µg/ml, GAM-APC, red-filled) from erythrocytes unstained by primary antibody (GAM-APC, black-dashed) in flow cytometry analysis (surface staining).
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