This antibody recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1 and 2, A antigens with chain types 3, 4, 5, 6) and Forssman antigen.

Applications

IHC-P, AGG

Reactivity

Human

Immunogen

Mixture of erythrocytes of group A1 and glycoprotein fraction isolated from saliva of secretors with blood group A.

Host

Mouse

Clonality

Monoclonal

Clone ID

HE-193

Isotype

IgM

Conjugate

Unconjugated

Purification

Concentrated by membrane ultrafiltration.

Purity

Tissue culture supernatant.

Product Form

Liquid

Formulation

Supplied as an aliquot of concentrated tissue culture supernatant.

Storage

Shipped at 4°C. Store at 2-8°C. Do not freeze!

Isotype Controls

Mouse IgM [PRF-03] (A86761)

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Citations for this Clone (1)

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Murine monoclonal antibodies to human A erythrocytes: differential reactivity with N-acetyl-D-galactosamine.

References: Anti-Blood Group A Antigen Antibody [HE-193] (ab2521)

Abstract:

Six different hybridoma cell lines were obtained producing IgM monoclonal antibodies (MAbs) against human blood group A antigen. All these MAbs agglutinate the A1, A1B and A2 erythrocytes, while only one of them agglutinates the A2B erythrocytes. The agglutination activity of 3 of these MAbs is inhibited by N-acetyl-D-galactosamine (D-GalNAc). Dissociation constants of complexes of these MAbs with D-GalNAc are approximately 2.2 X 10(-3) mol/l.

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