Our blog contains the latest product information and resources, including: FAQ's, pathways, trick tips, and protocols.
Dr Rachel StewartAug 15, 2020
Dr Rachel Stewart
Aug 15, 2020
Western blotting relies on the electrophoretic separation of proteins from a complex mixture based on their mass, the transfer of these proteins to a solid matrix, and the detection of specific proteins of interest on the matrix using antibodies. This article provides a detailed overview of western blotting, including: sample preparation, gel electrophoresis, protein transfer, immunodetection, sample protocols, buffers and reagents, and troubleshooting guide.
Dr Francesco PaduanoAug 15, 2020
Dr Francesco Paduano
Aug 15, 2020
A recombinant antibody is a synthetic monoclonal antibody that is generated by recombinant DNA technology; an in vitro alternative to the traditional hybridoma-based technology for creating monoclonal antibodies. This article introduces recombinant antibodies, explains how they are made, and explores their advantages compared to traditional monoclonal antibodies.
Dr Rachel StewartAug 15, 2020
Dr Rachel Stewart
Aug 15, 2020
The cell nucleus is the largest and quite often the stiffest organelle in a cell, and is a complex and dynamic structure that supports a surprisingly diverse set of functions, including: genome organization, transcriptional regulation, DNA damage repair, and mechanosensing. Learn about the cellular and molecular biology of the nucleus and the most popular nuclear markers.
Dr Laura NortonAug 15, 2020
Dr Laura Norton
Aug 15, 2020
The insulin signaling pathway is a biochemical cascade where by insulin increases the uptake of glucose into muscle and fat cells and reduces the synthesis of glucose in the liver in order to maintain glucose homeostasis. Learn about the insulin signaling pathway and how insulin affects glucose storage, glucose uptake, and protein lipid synthesis through Ras, Akt, mTor and MAPK.
Dr Francesco PaduanoAug 15, 2020
Dr Francesco Paduano
Aug 15, 2020
We offer a large range of isotype control antibodies that can be used to determine the level of background staining due to non-specific antibody binding. This article introduces isotype controls and explains why they are important, how to choose an isotype control, what experimental conditions to consider, and lists our recommended isotype controls.
Dr Stewart NewloveAug 15, 2020
Dr Stewart Newlove
Aug 15, 2020
We offer reagents that can be used for studying or testing: SARS-CoV-2 (2019-nCoV), SARS-CoV, MERS-CoV, 229E, NL63, OC43, and HKU1. Products include antibodies specific for human coronavirus, recombinant coronavirus proteins, cytokine detection products, viral RNA extraction kits, and RT-PCR reagents.
Dr Stewart NewloveAug 15, 2020
Dr Stewart Newlove
Aug 15, 2020
Loading control antibodies are used to confirm that protein loading is the same across a gel. This is essential in order to determine whether the difference in levels of the protein of interest is due to loading variance or sample-to-sample expression level differences. This article introduces the common loading controls for each sample type and list our recommended isotype control antibodies.
Dr Stewart NewloveAug 15, 2020
Dr Stewart Newlove
Aug 15, 2020
Epitope tags are short peptide sequences, generally composed of 10-15 amino acids, designed to create a molecular handle for a protein. By introducing a unique sequence of amino acids into a recombinant protein, antibodies against the corresponding epitope tag can be used to track protein expression and visualise the protein in tissues, cells, and organelles.
Dr Stewart NewloveAug 15, 2020
Dr Stewart Newlove
Aug 15, 2020
As a company of scientists, we understand how important it is to work with products that have been validated for specificity, activity, stability, and performance. All of the products in our catalogue have been validated in multiple research applications and with samples derived from multiple species.
Dr Stewart NewloveAug 15, 2020
Dr Stewart Newlove
Aug 15, 2020
Detailed product citations help the scientific research community in two ways. Firstly, they make it easy for other scientists to procure the same reagents in order to reproduce the results themselves. Secondly, they act as independent validation that a reagent works in a particular application and with samples derived from a particular species. This article provides a guide to help you properly cite antibodies.
Dr Stewart NewloveAug 15, 2020
Dr Stewart Newlove
Aug 15, 2020
Antibodies are widely used in pre-clinical research to study proteins and their functions in biological pathways and diseased states, however, a growing number of high-impact studies and publications have shown that not all antibodies are specific; contributing to the increasing amount of irreproducible pre-clinical research. Antibodies.com has incorporated knockout validation using CRISPR/Cas9, the most accepted method for validating antibody specificity, as part of its standard antibody validation process.
![Flow Cytometry - Mouse IgG1 [MOPC-21] (APC-Cyanine 7) (A122148) - Antibodies.com](https://cdn.antibodies.com/image/catalog/Antibodies/A122148_1.jpg?profile=blog_home_images)
![Western Blot - Anti-beta Tubulin Antibody [BT7R] (A85273) - Antibodies.com](https://cdn.antibodies.com/image/catalog/Antibodies/A85273_1.png?profile=blog_home_images)
