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BLNK (phospho Tyr84) Cell Based ELISA Kit (A102451)

Western Blot - BLNK (phospho Tyr84) Cell Based ELISA Kit (CBP1783) - Antibodies.com Protocol Diagram - BLNK (phospho Tyr84) Cell Based ELISA Kit (CBP1783) - Antibodies.com Protocol Summary - BLNK (phospho Tyr84) Cell Based ELISA Kit (CBP1783) - Antibodies.com Western Blot - Control Antibody - BLNK (phospho Tyr84) Cell Based ELISA Kit (CBP1783) - Antibodies.com
$645
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$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Email
orders@antibodies.com

Name
BLNK (phospho Tyr84) Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This BLNK (phospho Tyr84) Cell Based ELISA Kit allows for the detection of BLNK (phospho Tyr84) and the effects that certain stimulation conditions have on BLNK (phospho Tyr84) expression in different cell lines. Qualitative determination of BLNK (phospho Tyr84) concentration is achieved by an indirect ELISA format. In essence, the BLNK (phospho Tyr84) is captured by Anti-BLNK (phospho Tyr84) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this BLNK (phospho Tyr84) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-BLNK Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse, Rat
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
AGM4, B cell adapter containing SH2 domain protein, B cell adapter containing Src homology 2 domain protein, B cell adaptor containing SH2 domain, B cell linker, B cell linker protein, B cell-specific adaptor protein, B-cell activation, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, B-cell linker protein, BASH, Bca, BLNK s, BLNK_HUMAN, Cytoplasmic adapter protein, Ly 57, Ly57, Lymphocyte antigen 57, Lyw 57, Lyw57, MGC111051, SH2 domain-containing leukocyte protein, 65-KD, SLP 65, SLP-65, SLP65, Src homology 2 domain containing leukocyte protein of 65 kDa, Src homology 2 domain-containing leukocyte protein of 65 kDa, Src homology [SH2] domain-containing leukocyte protein of 65 kD
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - BLNK (phospho Tyr84) Cell Based ELISA Kit (CBP1783) - Antibodies.comEnlarge Image
Figure 1: Western Blot - BLNK (phospho Tyr84) Cell Based ELISA Kit (A102451)
Western blot analysis of extracts from K562 cells treated with starved 24h, using Anti-BLNK (phopho Tyr84) Antibody.
Protocol Diagram - BLNK (phospho Tyr84) Cell Based ELISA Kit (CBP1783) - Antibodies.comEnlarge Image
Figure 2: BLNK (phospho Tyr84) Cell Based ELISA Kit (A102451)
A diagram showing the protocol for BLNK (phospho Tyr84) Cell Based ELISA Kit.
Protocol Summary - BLNK (phospho Tyr84) Cell Based ELISA Kit (CBP1783) - Antibodies.comEnlarge Image
Figure 3: BLNK (phospho Tyr84) Cell Based ELISA Kit (A102451)
A summary of the protocol for BLNK (phospho Tyr84) Cell Based ELISA Kit.
Western Blot - Control Antibody - BLNK (phospho Tyr84) Cell Based ELISA Kit (CBP1783) - Antibodies.comEnlarge Image
Figure 4: Western Blot - BLNK (phospho Tyr84) Cell Based ELISA Kit (A102451)
The mouse monoclonal antibody to GAPDH is used as a positive control inBLNK (phospho Tyr84) Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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