This kit is for the biotinylation of primary antibodies.
Applications
IHC, ICC/IF, Flow Cytometry, ELISA, WB, IP
Principle of Assay
The Biotinylation Kit an efficient method of generating highly pure biotinylated antibodies. Your chosen antibody is first mixed with the reaction buffer and immobilized to resin within a spin column. The biotinylation reagent is then added and the antibody biotinylation reaction takes place on the resin. After washing, the pure biotinylated antibody is eluted from the resin, with the whole process taking approximately 1 hour. The kit is compatible with antibodies from any host species.