The antibody detects endogenous levels of BIK only when phosphorylated at threonine 33.
Applications
WB, IHC
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of threonine 33 (G-M-T(p)-D-S) derived from Human BIK.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from K562 cells, using BIK (Phospho-Thr33) antibody #12131. The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using BIK (Phospho-Thr33) antibody (#A0053) #12131. The picture on the right is treated with the synthesized peptide.
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