Anti-BIG-2 Antibody (A82948)

Name: Anti-BIG-2 Antibody
Brand: Antibodies.com
SKU: A82948
Price: 480.0 USD
Availability: InStock

This product is identical to Thermo (PA5-37991). Discover more for less.

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This product is discontinued

Anti-BIG-2 Antibody (A82948) has been discontinued and is no longer available.

View all Anti-BIG-2 Antibodies.

Specifications

Name

Anti-BIG-2 Antibody

Description

Goat polyclonal antibody to BIG-2.

Applications

ELISA, WB

Dilutions

ELISA: 1:128,000, WB: 0.5-1.5µg/ml

Reactivity

Mouse

Immunogen

Synthetic peptide corresponding to the internal region, amino acids 160-172, of mouse BIG-2.

Sequence

C-RRADGKPIARK

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

150 kDa

Predicted MW

113kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

AXCAM, Axonal associated cell adhesion molecule, BIG 2, Brain derived immunoglobulin superfamily protein 2, Brain-derived immunoglobulin superfamily protein 2, CNTN4, CNTN4A, CNTN4_HUMAN, contactin 4, Contactin-4, Neural cell adhesion protein BIG 2, SCA16

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (1)

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Figure 1: Western Blot - Anti-BIG-2 Antibody (A82948)

BIG-2 expression in Mouse Olfactory bulb (lanes 1 + 3) and Cerebral cortex (lanes 2 + 4) analyzed by western blot. Wildtype (lane 3 + 4) and knockout (lane 1+ 2) are compared. HEK293 cells were transfected to overexpress Mouse Cntn4 (last lane). Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-BIG-2 Antibody (A82948) at 0.5µg/ml for 1 hour and detected with chemiluminescence.

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