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Anti-beta Tubulin Antibody [BT7R] (A85273)

Western Blot - Anti-ß-Tubulin Antibody (A85273) - Antibodies.com Western Blot - Anti-ß-Tubulin Antibody (A85273) - Antibodies.com
$195
Product Datasheet

Mouse monoclonal (BT7R) antibody tobeta Tubulin for Dot, ELISA, IS and WB.

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A86730
Anti-beta Tubulin Antibody [TU-13]
Mouse monoclonal [TU-13] antibody to beta Tubulin for WB, IHC-Fr and ICC.
Name
Anti-beta Tubulin Antibody [BT7R]
Description
Mouse monoclonal (BT7R) antibody tobeta Tubulin.
Specificity
Recognizes native and denatured forms of ß-Tubulin (~50kDa).
Applications
Dot, ELISA, IS, WB
Reactivity
Chicken, Human, Monkey, Mouse, Rat, Rabbit
Immunogen
ß-Tubulin N-terminal peptide-KLH conjugates.
Host
Mouse
Clonality
Monoclonal
Clone ID
BT7R
Isotype
IgG2a
Conjugate

Unconjugated

Purification
Protein A affinity chromatography from mouse ascites fluid.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline, pH 7.20, with 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Beta 4 tubulin, Beta 5 tubulin, beta Ib tubulin, Beta1 tubulin, Class I beta tubulin, M40, MGC117247, MGC16435, OK/SW cl.56, OK/SWcl.56, TBB5_HUMAN, TUBB, TUBB 1, TUBB 2, TUBB 5, TUBB1, TUBB2, TUBB5, tubulin beta 1 chain, Tubulin beta 2 chain, tubulin beta 5 chain, Tubulin beta chain, Tubulin beta class I, tubulin beta polypeptide, Tubulin beta-5 chain
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-ß-Tubulin Antibody (A85273) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-beta Tubulin Antibody [BT7R] (A85273)
Three of Antibodies.com loading control mAbs reacting against 10µg/lane of mouse brain tissue lysates. 50kDa band is Anti-ß-Tubulin (BT7R) at 1:2000 dilution (0.5µg/mL); 42kDa band is Anti-ß-Actin (BA3R) at 1:1000 dilution (1µg/mL); 37kDa band is Anti-GAPDH (GA1R) at 1:5000 dilution (0.2µg/mL).
Western Blot - Anti-ß-Tubulin Antibody (A85273) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-beta Tubulin Antibody [BT7R] (A85273)
1:4000 (0.25µg/mL) Ab dilution used in WB of 10µg/lane (1), 5µg/lane (2), 2.5µg/lane (3), 1.25µg/lane (4) mouse brain tissue lysates.
Publishing research using Anti-beta Tubulin Antibody [BT7R] (A85273)? Please let us know so that we can list the citation on this page.

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