Anti-beta Tubulin Antibody [ARC0203] (A306704)

Name: Anti-beta Tubulin Antibody [ARC0203]
Brand: Antibodies.com
SKU: A306704
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0203] antibody to beta Tubulin for WB, ICC/IF and IP.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-beta Tubulin Antibody [ARC0203]

Description

Rabbit monoclonal [ARC0203] antibody to beta Tubulin.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human ß-Tubulin (P07437).

Sequence

LRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0203

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Beta 4 tubulin, Beta 5 tubulin, beta Ib tubulin, Beta1 tubulin, Class I beta tubulin, M40, MGC117247, MGC16435, OK/SW cl.56, OK/SWcl.56, TBB5_HUMAN, TUBB, TUBB 1, TUBB 2, TUBB 5, TUBB1, TUBB2, TUBB5, tubulin beta 1 chain, Tubulin beta 2 chain, tubulin beta 5 chain, Tubulin beta chain, Tubulin beta class I, tubulin beta polypeptide, Tubulin beta-5 chain

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-beta Tubulin Antibody [ARC0203] (A306704)

Western blot analysis of extracts of various cell lines, using Anti-beta Tubulin Antibody [ARC0203] (A306704) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Immunofluorescence - Anti-beta Tubulin Antibody [ARC0203] (A306704)

Immunofluorescence analysis of C6 cells using Anti-beta Tubulin Antibody [ARC0203] (A306704) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-beta Tubulin Antibody [ARC0203] (A306704)

Immunofluorescence analysis of NIH/3T3 cells using Anti-beta Tubulin Antibody [ARC0203] (A306704) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-beta Tubulin Antibody [ARC0203] (A306704)

Immunofluorescence analysis of U2OS cells using Anti-beta Tubulin Antibody [ARC0203] (A306704) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).