Anti-beta III Tubulin Antibody (A90524)

Name: Anti-beta III Tubulin Antibody
Brand: Antibodies.com
SKU: A90524
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to beta III Tubulin for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Knockout Validated Antibody Cytoskeleton Marker Microtubule Marker Neuronal Marker

Specifications

Name

Anti-beta III Tubulin Antibody

Description

Rabbit polyclonal antibody to beta III Tubulin.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human ßIII-Tubulin/ß3-Tubulin.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

TUBB3, TUBB4, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-beta III Tubulin Antibody (A90524)

Western blot analysis of extracts of various cell lines, using Anti-beta III Tubulin Antibody (A90524) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Western Blot - Anti-beta III Tubulin Antibody (A90524)

Western blot analysis of extracts from normal (control) and betaIII-Tubulin/beta3-Tubulin Rabbit polyclonal antibody knockout (KO) HeLa cells, using Anti-beta III Tubulin Antibody (A90524) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunofluorescence - Anti-beta III Tubulin Antibody (A90524)

Immunofluorescence analysis of mouse brain using Anti-beta III Tubulin Antibody (A90524) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-beta III Tubulin Antibody (A90524)

Immunofluorescence analysis of rat brain using Anti-beta III Tubulin Antibody (A90524) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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