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Anti-beta Catenin (phospho Ser552) Antibody (A11057)

Western Blot - Anti-beta Catenin (phospho Ser552) Antibody (A11057) - Antibodies.com Western Blot - Anti-beta Catenin (phospho Ser552) Antibody (A11057) - Antibodies.com
$435
Product Datasheet

Rabbit polyclonal antibody to beta Catenin (phospho Ser552) for WB.

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Name
Anti-beta Catenin (phospho Ser552) Antibody
Description
Rabbit polyclonal antibody to beta Catenin (phospho Ser552).
Applications
WB
Dilutions
WB: 1:500-1:2,000
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphorylated peptide around S552 of human beta Catenin (NP_001091679).
Sequence
RTSMG
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
106 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Beta-catenin, Cadherin associated protein, Catenin (cadherin associated protein), beta 1, 88kDa, Catenin beta 1, Catenin beta-1, CATNB, CHBCAT, CTNB1_HUMAN, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, OTTHUMP00000162082, OTTHUMP00000165222, OTTHUMP00000165223, OTTHUMP00000209288, OTTHUMP00000209289
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-beta Catenin (phospho Ser552) Antibody (A11057) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-beta Catenin (phospho Ser552) Antibody (A11057)
Western blot analysis of extracts of various cell lines, using Anti-beta Catenin (phospho Ser552) Antibody (A11057) at 1:1,000 dilution. C2C12 cell lysates were treated by CIP at 37°C for 1 hour. NIH/3T3 cells were treated by Forskolin (30uM) for 30 minutes after serum-starvation overnight or treated by CIP at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 30s.
Western Blot - Anti-beta Catenin (phospho Ser552) Antibody (A11057) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-beta Catenin (phospho Ser552) Antibody (A11057)
Western blot analysis of extracts of C2C12 cells, using Anti-beta Catenin (phospho Ser552) Antibody (A11057) at 1:1,000 dilution or beta-Catenin antibody (A11932). C2C12 cell lysates were treated by CIP at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 30s.
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