Western Blot - Anti-beta Catenin Antibody (A83637)
beta Catenin expression in HeLa cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-beta Catenin Antibody (A83637) at 0.3µg/ml and detected by chemiluminescence.
beta Catenin expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-beta Catenin Antibody (A83637) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Cytoplasmic/plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 4µg/ml.
beta Catenin expression in Human Adrenal Gland analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-beta Catenin Antibody (A83637) at 5µg/ml and revealed with alkaline phosphatase (AP).
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