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Anti-beta Catenin Antibody (A80679)

Western Blot - Anti-beta Catenin Antibody (A80679) - Antibodies.com Western Blot - Anti-beta Catenin Antibody (A80679) - Antibodies.com Western Blot - Anti-beta Catenin Antibody (A80679) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to beta Catenin for WB, IHC and IP.

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5-8 Business Days

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Mouse monoclonal [EM-22] antibody to beta Catenin for IP, WB, ICC and Flow Cytometry (Intracellular).
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Anti-beta Catenin Antibody [EM-22]
Mouse monoclonal [EM-22] antibody to beta Catenin for IP, WB, ICC and Flow Cytometry (Intracellular).
Name
Anti-beta Catenin Antibody
Description
Rabbit polyclonal antibody to beta Catenin.
Applications
WB, IHC, IP
Dilutions
WB: 1:100-1:500, IHC: 1:20-1:200, IP: 1:500-1:1,000
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ß-Catenin (NP_001895.1).
Sequence
MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFP
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
92 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Beta-catenin, Cadherin associated protein, Catenin (cadherin associated protein), beta 1, 88kDa, Catenin beta 1, Catenin beta-1, CATNB, CHBCAT, CTNB1_HUMAN, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, OTTHUMP00000162082, OTTHUMP00000165222, OTTHUMP00000165223, OTTHUMP00000209288, OTTHUMP00000209289
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-beta Catenin Antibody (A80679) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-beta Catenin Antibody (A80679)
Western blot analysis of extracts of various cell lines, using Anti-beta Catenin Antibody (A80679) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Western Blot - Anti-beta Catenin Antibody (A80679) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-beta Catenin Antibody (A80679)
Western blot analysis of extracts of 293T cells, using Anti-beta Catenin Antibody (A80679) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Western Blot - Anti-beta Catenin Antibody (A80679) - Antibodies.comEnlarge Image
Figure 3: Western Blot - Anti-beta Catenin Antibody (A80679)
Immunoprecipitation analysis of 300µg extracts of 293T cells using 3µg of Anti-beta Catenin Antibody (A80679). This Western blot was performed on the immunoprecipitate using Anti-beta Catenin Antibody (A80679) at a dilution of 1:1000.
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