Anti-beta Arrestin 1 Antibody (A12755)

Name: Anti-beta Arrestin 1 Antibody
Brand: Antibodies.com
SKU: A12755
Price: 345.0 USD
Availability: InStock

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$345

Rabbit polyclonal antibody to beta Arrestin 1 for WB, IHC, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-beta Arrestin 1 Antibody

Description

Rabbit polyclonal antibody to beta Arrestin 1.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 169-418 of human ß-arrestin1 (NP_004032.2).

Sequence

RKVQYAPERPGPQPTAETTRQFLMSDKPLHLEASLDKEIYYHGEPISVNVHVTNNTNKTVKKIKISVRQYADICLFNTAQYKCPVAMEEADDTVAPSSTFCKVYTLTPFLANNREKRGLALDGKLKHEDTNLASSTLLREGANREILGIIVSYKVKVKLVVSRGGLLGDLASSDVAVELPFTLMHPKPKEEPPHREVPENETPVDTNLIELDTNDDDIVFEDFARQRLKGMKDDKEEEEDGTGSPQLNNR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

51 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ARR1, ARRB1, Arrestin beta-1, Beta-arrestin-1, Non-visual arrestin-2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (8)

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Western Blot - Anti-beta Arrestin 1 Antibody (A12755)

Western blot analysis of extracts of various cell lines, using Anti-beta Arrestin 1 Antibody (A12755) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunohistochemistry - Anti-beta Arrestin 1 Antibody (A12755)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-beta Arrestin 1 Antibody (A12755) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-beta Arrestin 1 Antibody (A12755)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-beta Arrestin 1 Antibody (A12755) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-beta Arrestin 1 Antibody (A12755)

Immunofluorescence analysis of A-549 cells using Anti-beta Arrestin 1 Antibody (A12755) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).