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Anti-beta Actin Antibody (A17300)

Western Blot - Anti-beta Actin Antibody (A17300) - Antibodies.com
$230
Product Datasheet

Rabbit polyclonal antibody to beta Actin for WB.

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Anti-beta Actin Antibody [S12-I]
Rabbit monoclonal (S12-I) antibody to beta Actin for WB, ELISA, IP and ICC.
Name
Anti-beta Actin Antibody
Description
Rabbit polyclonal antibody to beta Actin.
Applications
WB
Dilutions
WB: 1:500-1:1,000
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-375 of human ß-Actin (NP_001092.1).
Sequence
MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
42 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
A26C1A, A26C1B, ACTB, ACTB_HUMAN, Actin beta, Actin cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed, Actx, b actin, Beta cytoskeletal actin, Beta-actin, BRWS1, E430023M04Rik, MGC128179, PS1TP5 binding protein 1, PS1TP5BP1
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-beta Actin Antibody (A17300) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-beta Actin Antibody (A17300)
Western blot analysis of extracts of various cell lines, using Anti-beta Actin Antibody (A17300) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Publishing research using Anti-beta Actin Antibody (A17300)? Please let us know so that we can list the citation on this page.

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