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Anti-BCS1L Antibody (A15822)

Western Blot - Anti-BCS1L Antibody (A15822) - Antibodies.com Immunohistochemistry - Anti-BCS1L Antibody (A15822) - Antibodies.com Immunohistochemistry - Anti-BCS1L Antibody (A15822) - Antibodies.com Immunofluorescence - Anti-BCS1L Antibody (A15822) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to BCS1L for WB, IHC and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-BCS1L Antibody
Description
Rabbit polyclonal antibody to BCS1L.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 33-240 of human BCS1L (NP_004319.1).
Sequence
RKGVQLGLVAFRRHYMITLEVPARDRSYAWLLSWLTRHSTRTQHLSVETSYLQHESGRISTKFEFVPSPGNHFIWYRGKWIRVERSREMQMIDLQTGTPWESVTFTALGTDRKVFFNILEEARELALQQEEGKTVMYTAVGSEWRPFGYPRRRRPLNSVVLQQGLADRIVRDVQEFIDNPKWYTDRGIPYRRGYLLYGPPGCGKSSFI
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
48 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
BCS, BCS 1, BCS 1L, BCS1, BCS1 (yeast homolog) like, BCS1 like, BCS1 like (S. cerevisiae), BCS1 like protein, Bjornstad syndrome (pili torti and deafness), BJS, FLNMS, GRACILE, h BCS, H BCS1, Hs.6719, Mitochondrial chaperone BCS1, Mitochondrial complex III assembly, PTD
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-BCS1L Antibody (A15822) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-BCS1L Antibody (A15822)
Western blot analysis of extracts of various cell lines, using Anti-BCS1L Antibody (A15822) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-BCS1L Antibody (A15822) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-BCS1L Antibody (A15822)
Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-BCS1L Antibody (A15822) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-BCS1L Antibody (A15822) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-BCS1L Antibody (A15822)
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-BCS1L Antibody (A15822) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-BCS1L Antibody (A15822) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-BCS1L Antibody (A15822)
Immunofluorescence analysis of U2OS cells using Anti-BCS1L Antibody (A15822) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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