Recombinant rabbit monoclonal [DM4] antibody to BCMA.
Applications
Flow Cytometry, MM Tumor cell killing
Dilutions
Flow Cytometry: 1:100
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Clone ID
DM4
Isotype
scFv
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Synonyms
B cell maturation antigen, B cell maturation factor, B cell maturation protein, B-cell maturation protein, BCM, CD269, CD269 antigen, TNFRSF17, TNR17_HUMAN, Tumor necrosis factor receptor superfamily member 17
The basic principle of BiTE cell killing assay. The BiTE molecule can effectively bring T cells to tumor target cells and stimulate tumor cell killing activity of T cells.
A. The scheme of Anti-BCMA BiTE molecule. B. Tumor cell killing assay. NCI-H929 cells (stably transfected with luciferase), were incubated with freshly isolated Human PBMC, and different concentration of BiTE antibodies constructed from Anti-BCMA Antibody [DM4] - Azide free (A318802) (red line) or BB2121 originated huC11D5.3 clone (blue line) or a no BCMA binding clone as negative control (black line). After 48hrs incubation, the viable NCI-H929 tumor cells were measured by luciferase activity assay.
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