Recombinant rabbit monoclonal [DM16] antibody to BCMA.
Applications
ELISA, Flow Cytometry, IF, IP
Dilutions
Flow Cytometry: 1:100, IP: 1:30
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Clone ID
DM16
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Synonyms
B cell maturation antigen, B cell maturation factor, B cell maturation protein, B-cell maturation protein, BCM, CD269, CD269 antigen, TNFRSF17, TNR17_HUMAN, Tumor necrosis factor receptor superfamily member 17
Detection of BCMA in K562-BCMA (K562 cells transduced with gene for full length BCMA) Human Cell Line or NCI-H929 Human Cell Line with Anti-BCMA Antibody [DM16] - Azide free (A318668) by Flow Cytometry.
Binding of different clones Rabbit Anti-Human BCMA/TNFRSF 17 Antigen Affinity-purified monoclonal antibody to NCI-H929 and K562-BCMA cells was determined by flow cytometry.
ELISA plate was coated with Recombinant Human BCMA Protein (Fc Chimera 6xHis Tag), followed by pre-blocking with Anti-BCMA Antibody [DM16] - Azide free (Grey bar) or rabbit control IgG (Black bar), and then different Rabbit Antibodies were added to check the competitive inhibition of Recombinant Human BCMA Protein (Fc Chimera 6xHis Tag). DM3 clone exhibits the strongest inhibition (Red bar). This data indicated that DM3 bind to the same epitope as bb2121.
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