Western Blot - Anti-Bcl-2 (phospho Ser70) Antibody (A11053)
Western blot analysis of extracts of Jurkat and 293 cells, using Anti-Bcl-2 (phospho Ser70) Antibody (A11053) at 1:1,000 dilution. Jurkat cells were treated by serum-starvation overnight. 293 cells were treated by UV for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.
Immunofluorescence analysis of HeLa cells using Anti-Bcl-2 (phospho Ser70) Antibody (A11053) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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