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BCL-2 Cell Based ELISA Kit (A103040)

Western Blot - BCL-2 Cell Based ELISA Kit (CB5068) - Antibodies.com Protocol Diagram - BCL-2 Cell Based ELISA Kit (CB5068) - Antibodies.com Protocol Summary - BCL-2 Cell Based ELISA Kit (CB5068) - Antibodies.com Western Blot - Control Antibody - BCL-2 Cell Based ELISA Kit (CB5068) - Antibodies.com
$550
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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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orders@antibodies.com

Name
BCL-2 Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This BCL-2 Cell Based ELISA Kit allows for the detection of BCL-2 and the effects that certain stimulation conditions have on BCL-2 expression in different cell lines. Qualitative determination of BCL-2 concentration is achieved by an indirect ELISA format. In essence, the BCL-2 is captured by Anti-BCL-2 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this BCL-2 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse, Rat
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
Apoptosis regulator Bcl 2, Apoptosis regulator Bcl-2, Apoptosis regulator Bcl2, AW986256, B cell CLL/lymphoma 2, B cell leukemia/lymphoma 2, Bcl2, BCL2_HUMAN, C430015F12Rik, D630044D05Rik, D830018M01Rik, Leukemia/lymphoma, B-cell, 2, Oncogene B-cell leukemia 2, PPP1R50, Protein phosphatase 1, regulatory subunit 50
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - BCL-2 Cell Based ELISA Kit (CB5068) - Antibodies.comEnlarge Image
Figure 1: Western Blot - BCL-2 Cell Based ELISA Kit (A103040)
Western blot analysis of extracts from MCF7 cells, treated with Etoposide, using Anti-BCL-2 (Ab-56) Antibody.
Protocol Diagram - BCL-2 Cell Based ELISA Kit (CB5068) - Antibodies.comEnlarge Image
Figure 2: BCL-2 Cell Based ELISA Kit (A103040)
A diagram showing the protocol for BCL-2 Cell Based ELISA Kit.
Protocol Summary - BCL-2 Cell Based ELISA Kit (CB5068) - Antibodies.comEnlarge Image
Figure 3: BCL-2 Cell Based ELISA Kit (A103040)
A summary of the protocol for BCL-2 Cell Based ELISA Kit.
Western Blot - Control Antibody - BCL-2 Cell Based ELISA Kit (CB5068) - Antibodies.comEnlarge Image
Figure 4: Western Blot - BCL-2 Cell Based ELISA Kit (A103040)
The mouse monoclonal antibody to GAPDH is used as a positive control inBCL-2 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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