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Anti-Bcl-2 Antibody [ARC0173] (A306910)

Western Blot - Anti-Bcl-2 Antibody [ARC0173] (A306910) - Antibodies.com Immunohistochemistry - Anti-Bcl-2 Antibody [ARC0173] (A306910) - Antibodies.com Immunohistochemistry - Anti-Bcl-2 Antibody [ARC0173] (A306910) - Antibodies.com Immunohistochemistry - Anti-Bcl-2 Antibody [ARC0173] (A306910) - Antibodies.com Immunohistochemistry - Anti-Bcl-2 Antibody [ARC0173] (A306910) - Antibodies.com Immunohistochemistry - Anti-Bcl-2 Antibody [ARC0173] (A306910) - Antibodies.com Western Blot - Anti-Bcl-2 Antibody [ARC0173] (A306910) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC0173] antibody to Bcl-2 for WB, IHC and IP.

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A86278
16 Citations
Anti-Bcl-2 Antibody [Bcl-2/100]
Mouse monoclonal [Bcl-2/100] antibody to Bcl-2 for Flow Cytometry, IP, WB, IHC-P, IHC-Fr and ICC.
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A86278
16 Citations
Anti-Bcl-2 Antibody [Bcl-2/100]
Mouse monoclonal [Bcl-2/100] antibody to Bcl-2 for Flow Cytometry, IP, WB, IHC-P, IHC-Fr and ICC.
Name
Anti-Bcl-2 Antibody [ARC0173]
Description
Rabbit monoclonal [ARC0173] antibody to Bcl-2.
Applications
WB, IHC, IP
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human Bcl-2 (P10415).
Sequence
MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPAPGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDNIALWMTEYLNRHLHTWIQDNGGWDAFVELYGPSMRPLFD
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC0173
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
26 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Apoptosis regulator Bcl 2, Apoptosis regulator Bcl-2, Apoptosis regulator Bcl2, AW986256, B cell CLL/lymphoma 2, B cell leukemia/lymphoma 2, Bcl2, BCL2_HUMAN, C430015F12Rik, D630044D05Rik, D830018M01Rik, Leukemia/lymphoma, B-cell, 2, Oncogene B-cell leukemia 2, PPP1R50, Protein phosphatase 1, regulatory subunit 50
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Bcl-2 Antibody [ARC0173] (A306910) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Bcl-2 Antibody [ARC0173] (A306910)
Western blot analysis of extracts of various cell lines, using Anti-Bcl-2 Antibody [ARC0173] (A306910) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry - Anti-Bcl-2 Antibody [ARC0173] (A306910) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-Bcl-2 Antibody [ARC0173] (A306910)
Immunohistochemistry analysis of paraffin-embedded human tonsil (100X) using Anti-Bcl-2 Antibody [ARC0173] (A306910) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Bcl-2 Antibody [ARC0173] (A306910) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-Bcl-2 Antibody [ARC0173] (A306910)
Immunohistochemistry analysis of paraffin-embedded human appendix tissue (100X) using Anti-Bcl-2 Antibody [ARC0173] (A306910) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Bcl-2 Antibody [ARC0173] (A306910) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-Bcl-2 Antibody [ARC0173] (A306910)
Immunohistochemistry analysis of paraffin-embedded human appendix tissue (400X) using Anti-Bcl-2 Antibody [ARC0173] (A306910) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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