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Anti-Bcl-2 Antibody (A16833)

Western Blot - Anti-Bcl-2 Antibody (A16833) - Antibodies.com Western Blot - Anti-Bcl-2 Antibody (A16833) - Antibodies.com Immunofluorescence - Anti-Bcl-2 Antibody (A16833) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Bcl-2 for WB, ICC/IF and IP.

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A86278
16 Citations
Anti-Bcl-2 Antibody [Bcl-2/100]
Mouse monoclonal [Bcl-2/100] antibody to Bcl-2 for Flow Cytometry, IP, WB, IHC-P, IHC-Fr and ICC.
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A86278
16 Citations
Anti-Bcl-2 Antibody [Bcl-2/100]
Mouse monoclonal [Bcl-2/100] antibody to Bcl-2 for Flow Cytometry, IP, WB, IHC-P, IHC-Fr and ICC.
Name
Anti-Bcl-2 Antibody
Description
Rabbit polyclonal antibody to Bcl-2.
Applications
WB, ICC/IF, IP
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:100, IP: 1:50-1:100
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-239 of human Bcl-2 (P10415).
Sequence
MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPAPGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDNIALWMTEYLNRHLHTWIQDNGGWDAFVELYGPSMRPLFDFSWLSLKTLLSLALVGACITLGAYLGHK
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
26 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Apoptosis regulator Bcl 2, Apoptosis regulator Bcl-2, Apoptosis regulator Bcl2, AW986256, B cell CLL/lymphoma 2, B cell leukemia/lymphoma 2, Bcl2, BCL2_HUMAN, C430015F12Rik, D630044D05Rik, D830018M01Rik, Leukemia/lymphoma, B-cell, 2, Oncogene B-cell leukemia 2, PPP1R50, Protein phosphatase 1, regulatory subunit 50
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Bcl-2 Antibody (A16833) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Bcl-2 Antibody (A16833)
Western blot analysis of extracts of Jurkat cells, using Anti-Bcl-2 Antibody (A16833) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Western Blot - Anti-Bcl-2 Antibody (A16833) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Bcl-2 Antibody (A16833)
Western blot analysis of extracts of Mouse lung, using Anti-Bcl-2 Antibody (A16833) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunofluorescence - Anti-Bcl-2 Antibody (A16833) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-Bcl-2 Antibody (A16833)
Immunofluorescence analysis of L929 cells using Anti-Bcl-2 Antibody (A16833) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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