Anti-BCAT1 Antibody (A91668)

Name: Anti-BCAT1 Antibody
Brand: Antibodies.com
SKU: A91668
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to BCAT1 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-BCAT1 Antibody

Description

Rabbit polyclonal antibody to BCAT1.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-190 of human BCAT1 (NP_005495.2).

Sequence

EVVGTFKAKDLIVTPATILKEKPDPNNLVFGTVFTDHMLTVEWSSEFGWEKPHIKPLQNLSLHPGSSALHYAVELFEGLKAFRGVDNKIRLFQPNLNMDRMYRSAVRATLPVFDKEELLECIQQLVKLDQEWVPYSTSASLYIRPTFIGTEPSLGVKKPTKALLFVLLSPV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

43 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BCAT, BCAT(c), BCAT1_HUMAN, BCT1, Branched chain amino acid aminotransferase, cytosolic, branched chain amino-acid transaminase 1, cytosolic, branched chain aminotransferase 1, branched chain aminotransferase 1, cytosolic, Branched-chain-amino-acid aminotransferase, cytosolic, DKFZp686E12175, EC 2.6.1.42, ECA39, MECA39, placental protein 18, PNAS 121, PP18, Protein ECA39

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-BCAT1 Antibody (A91668)

Western blot analysis of extracts of various cell lines, using Anti-BCAT1 Antibody (A91668) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunofluorescence - Anti-BCAT1 Antibody (A91668)

Immunofluorescence analysis of HeLa cells using Anti-BCAT1 Antibody (A91668) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-BCAT1 Antibody (A91668)

Immunofluorescence analysis of HepG2 cells using Anti-BCAT1 Antibody (A91668) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-BCAT1 Antibody (A91668)

Immunofluorescence analysis of NIH/3T3 cells using Anti-BCAT1 Antibody (A91668) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).