Anti-Bax Antibody [ARC5006-10] (A306010)

Name: Anti-Bax Antibody [ARC5006-10]
Brand: Antibodies.com
SKU: A306010
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC5006-10] antibody to Bax for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Knockout Validated Antibody

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Rabbit polyclonal antibody to BAX for IHC, IF and ELISA.

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Anti-BAX Antibody

Rabbit polyclonal antibody to BAX for IHC, IF and ELISA.

Specifications

Name

Anti-Bax Antibody [ARC5006-10]

Description

Rabbit monoclonal [ARC5006-10] antibody to Bax.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:2,000-1:10,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:100-1:500

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 10-70 of human Bax (NP_620116.1).

Sequence

GGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDEL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC5006-10

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

21 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Apoptosis regulator BAX, Bax-protein, BAXA, Baxdelta2G9, Baxdelta2G9omega, Baxdelta2omega, BAX_HUMAN, Bcl-2-like protein 4, BCL2 associated X protein, BCL2 associated X protein omega, BCL2 associated X protein transcript variant delta2, Bcl2-L-4, BCL2L4, membrane isoform alpha

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (9)

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Figure 1: Western Blot - Anti-Bax Antibody [ARC5006-10] (A306010)

Western blot analysis of various lysates, using Anti-Bax Antibody [ARC5006-10] (A306010) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-Bax Antibody [ARC5006-10] (A306010)

Western blot analysis of extracts from wild type(WT) and Bax knockout (KO) 293T cells, using Anti-Bax Antibody [ARC5006-10] (A306010) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-Bax Antibody [ARC5006-10] (A306010)

Immunohistochemistry analysis of paraffin-embedded rat lymph node using Anti-Bax Antibody [ARC5006-10] (A306010) at a dilution of 1:20ï¼Œ1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Bax Antibody [ARC5006-10] (A306010)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Bax Antibody [ARC5006-10] (A306010) at a dilution of 1:20ï¼Œ1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.