Anti-Bax Antibody [ARC0164] (A307133)

Name: Anti-Bax Antibody [ARC0164]
Brand: Antibodies.com
SKU: A307133
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0164] antibody to Bax for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Anti-BAX Antibody

Rabbit polyclonal antibody to BAX for IHC, IF and ELISA.

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A96467

Anti-BAX Antibody

Rabbit polyclonal antibody to BAX for IHC, IF and ELISA.

Specifications

Name

Anti-Bax Antibody [ARC0164]

Description

Rabbit monoclonal [ARC0164] antibody to Bax.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bax (Q07812).

Sequence

MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMF

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0164

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

21 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Apoptosis regulator BAX, Bax-protein, BAXA, Baxdelta2G9, Baxdelta2G9omega, Baxdelta2omega, BAX_HUMAN, Bcl-2-like protein 4, BCL2 associated X protein, BCL2 associated X protein omega, BCL2 associated X protein transcript variant delta2, Bcl2-L-4, BCL2L4, membrane isoform alpha

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Bax Antibody [ARC0164] (A307133)

Western blot analysis of extracts of various cell lines, using Anti-Bax Antibody [ARC0164] (A307133) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-Bax Antibody [ARC0164] (A307133)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-Bax Antibody [ARC0164] (A307133) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Bax Antibody [ARC0164] (A307133)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Bax Antibody [ARC0164] (A307133) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.