Anti-Bax Antibody (A81196)

Name: Anti-Bax Antibody
Brand: Antibodies.com
SKU: A81196
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to Bax for WB, IHC, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Bax Antibody

Description

Rabbit polyclonal antibody to Bax.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bax (NP_620116.1).

Sequence

MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMF

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

21 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4, BCL2L4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (7)

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Western Blot - Anti-Bax Antibody (A81196)

Western blot analysis of extracts of various cell lines, using Anti-Bax Antibody (A81196) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunohistochemistry - Anti-Bax Antibody (A81196)

Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-Bax Antibody (A81196) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Bax Antibody (A81196)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-Bax Antibody (A81196) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-Bax Antibody (A81196)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Bax Antibody (A81196) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).