Anti-Bax Antibody (A12539)

Name: Anti-Bax Antibody
Brand: Antibodies.com
SKU: A12539
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to Bax for WB, IHC, ICC/IF and IP.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Tags:

Knockout Validated Antibody

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A96467

Anti-BAX Antibody

Rabbit polyclonal antibody to BAX for IHC, IF and ELISA.

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A96467

Anti-BAX Antibody

Rabbit polyclonal antibody to BAX for IHC, IF and ELISA.

Specifications

Name

Anti-Bax Antibody

Description

Rabbit polyclonal antibody to Bax.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bax (NP_620116.1).

Sequence

MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMF

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

21 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Apoptosis regulator BAX, Bax-protein, BAXA, Baxdelta2G9, Baxdelta2G9omega, Baxdelta2omega, BAX_HUMAN, Bcl-2-like protein 4, BCL2 associated X protein, BCL2 associated X protein omega, BCL2 associated X protein transcript variant delta2, Bcl2-L-4, BCL2L4, membrane isoform alpha

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (9)

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Figure 1: Western Blot - Anti-Bax Antibody (A12539)

Western blot analysis of extracts from normal (control) and Bax knockout (KO) 293T cells, using Anti-Bax Antibody (A12539) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-Bax Antibody (A12539)

Western blot analysis of extracts of various cell lines, using Anti-Bax Antibody (A12539) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 3: Western Blot - Anti-Bax Antibody (A12539)

Western blot analysis of extracts of Mouse brain, using Anti-Bax Antibody (A12539) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 4: Immunohistochemistry - Anti-Bax Antibody (A12539)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-Bax Antibody (A12539) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.