Anti-Bag3 Antibody (A91265)

Name: Anti-Bag3 Antibody
Brand: Antibodies.com
SKU: A91265
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Bag3 for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Bag3 Antibody

Description

Rabbit polyclonal antibody to Bag3.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100, IP: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 380-575 of human BAG3 (NP_004272.2).

Sequence

PSAVPSSPKSVATEERAAPSTAPAEATPPKPGEAEAPPKHPGVLKVEAILEKVQGLEQAVDNFEGKKTDKKYLMIEEYLTKELLALDSVDPEGRADVRQARRDGVRKVQTILEKLEQKAIDVPGQVQVYELQPSNLEADQPLQAIMEMGAVAADKGKKNAGNAEDPHTETQQPEATAAATSNPSSMTDTPGNPAAP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

80 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BAG 3, BAG family molecular chaperone regulator 3, BAG-3, BAG3_HUMAN, Bcl 2 binding protein, Bcl-2-associated athanogene 3, Bcl-2-binding protein Bis, BCL2 associated athanogene 3, BCL2 binding athanogene 3, BIS, CAIR 1, Docking protein CAIR 1, Docking protein CAIR-1, MFM6

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Bag3 Antibody (A91265)

Western blot analysis of extracts of various cell lines, using Anti-Bag3 Antibody (A91265) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-Bag3 Antibody (A91265)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-Bag3 Antibody (A91265) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Bag3 Antibody (A91265)

Immunohistochemistry analysis of paraffin-embedded human lung using Anti-Bag3 Antibody (A91265) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Bag3 Antibody (A91265)

Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-Bag3 Antibody (A91265) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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