Anti-BACE1 Antibody (A14638)

Name: Anti-BACE1 Antibody
Brand: Antibodies.com
SKU: A14638
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to BACE1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-BACE1 Antibody

Description

Rabbit polyclonal antibody to BACE1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 22-322 of human BACE1 (NP_036236.1).

Sequence

TQHGIRLPLRSGLGGAPLGLRLPRETDEEPEEPGRRGSFVEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTYRDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHVPNLFSLQLCGAGFPLNQSEVLASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGF

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

APP beta secretase, Asp 2, ASP2, Aspartyl protease 2, BACE, BACE 1, BACE1_HUMAN, Beta secretase, Beta secretase 1, Beta site amyloid beta A4 precursor protein cleaving enzyme, Beta site amyloid precursor protein cleaving enzyme, Beta site amyloid precursor protein cleaving enzyme 1, Beta site APP cleaving enzyme, Beta site APP cleaving enzyme 1, Beta-secretase 1, Beta-site amyloid precursor protein cleaving enzyme 1, Beta-site APP cleaving enzyme 1, FLJ90568, HSPC104, Memapsin 2, Memapsin-2, Memapsin2, Membrane associated aspartic protease 2, Membrane-associated aspartic protease 2, Transmembrane aspartic proteinase Asp2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-BACE1 Antibody (A14638)

Western blot analysis of SH-SY5Y, using Anti-BACE1 Antibody (A14638) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-BACE1 Antibody (A14638)

Immunohistochemistry analysis of paraffin-embedded mouse hippocampus using Anti-BACE1 Antibody (A14638) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-BACE1 Antibody (A14638)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-BACE1 Antibody (A14638) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-BACE1 Antibody (A14638)

Immunofluorescence analysis of U-251MG cells using Anti-BACE1 Antibody (A14638) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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