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Anti-B-RAF (phospho Ser446) Antibody (A93880)

Western Blot - Anti-B-RAF (phospho Ser446) Antibody (A8082) - Antibodies.com Immunohistochemistry - Anti-B-RAF (phospho Ser446) Antibody (A8082) - Antibodies.com Western Blot - Anti-B-RAF (phospho Ser446) Antibody (A8082) - Antibodies.com Western Blot - Anti-B-RAF (phospho Ser446) Antibody (A8082) - Antibodies.com
$190
Product Datasheet

Rabbit polyclonal antibody to B-RAF (phospho Ser446) for WB, IHC and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 6-8 business days.

  • Specifications

Name
Anti-B-RAF (phospho Ser446) Antibody
Description
Rabbit polyclonal antibody to B-RAF (phospho Ser446).
Specificity
This antibody detects endogenous levels of B-RAF only when phosphorylated at Ser446.
Applications
WB, IHC, ELISA
Dilutions
WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:20000
Reactivity
Human, Mouse
Immunogen
Synthetic peptide derived from human B-RAF around the phosphorylation site of Ser446 (amino acids 411-460).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
84kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
BRAF, BRAF1, p94, Proto-oncogene B-Raf, RAFB1, Serine/threonine-protein kinase B-raf, v-Raf murine sarcoma viral oncogene homolog B1
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western Blot - Anti-B-RAF (phospho Ser446) Antibody (A8082) - Antibodies.comEnlarge Image

Western Blot - Anti-B-RAF (phospho Ser446) Antibody (A93880)

Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30' using Anti-B-RAF (phospho Ser446) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemistry - Anti-B-RAF (phospho Ser446) Antibody (A8082) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-B-RAF (phospho Ser446) Antibody (A93880)

Immunohistochemical analysis of paraffin-embedded human brain using Anti-B-RAF (phospho Ser446) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Western Blot - Anti-B-RAF (phospho Ser446) Antibody (A8082) - Antibodies.comEnlarge Image

Western Blot - Anti-B-RAF (phospho Ser446) Antibody (A93880)

Western blot analysis of 293 cells using Anti-B-RAF (phospho Ser446) Antibody.
Western Blot - Anti-B-RAF (phospho Ser446) Antibody (A8082) - Antibodies.comEnlarge Image

Western Blot - Anti-B-RAF (phospho Ser446) Antibody (A93880)

Western blot analysis of K562 293T lysis using Anti-B-RAF (phospho Ser446) Antibody.
Publishing research using Anti-B-RAF (phospho Ser446) Antibody (A93880)? Please let us know so that we can list the citation on this page.

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