Anti-Aurora A (phospho-Thr288) Antibody (A50385)

Name: Anti-Aurora A (phospho-Thr288) Antibody
Brand: Antibodies.com
SKU: A50385
Price: 265.0 USD
Availability: InStock

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$265

Rabbit polyclonal antibody to Aurora A (phospho-Thr288) for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Aurora A (phospho-Thr288) Antibody

Description

Rabbit polyclonal antibody to Aurora A (phospho-Thr288)

Specificity

The antibody detects endogenous level of Aurora A only when phosphorylated at threonine 288.

Applications

WB, IHC

Dilutions

Immunohistochemistry: 1:50~1:100

Reactivity

Human, Mouse

Immunogen

Peptide sequence around phosphorylation site of threonine 288 (R-T-T(p)-L-M) derived from Human Aurora A.

Host

Rabbit

Clonality

Polyclonal

Conjugate

Unconjugated

Purification

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1.0mg / ml

Predicted MW

48kd

Formulation

Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Synonyms

AIK, ARK-1, ARK1, AURA, Aurka, Aurora 2, Aurora kinase A, Aurora-related kinase 1, Aurora/IPL1 like kinase, Aurora/IPL1-related kinase 1, AURORA2, Breast tumor-amplified kinase, BTAK, hARK1, IAK, IPL1 related kinase, MGC34538, OTTHUMP00000031340, OTTHUMP00000031341, OTTHUMP00000031342, OTTHUMP00000031343, OTTHUMP00000031344, OTTHUMP00000031345, OTTHUMP00000166071, OTTHUMP00000166072, PPP1R47, Protein phosphatase 1, regulatory subunit 47, Serine/threonine kinase 15, Serine/threonine kinase 6, Serine/threonine-protein kinase 15, Serine/threonine-protein kinase 6, Serine/threonine-protein kinase aurora-A, STK15, STK6, STK6_HUMAN, STK7

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Immunohistochemistry - Anti-Aurora A (phospho-Thr288) Antibody (A50385)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Aurora A (Phospho-Thr288) Antibody #11519 (left) or the same antibody preincubated with blocking peptide (right).

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Figure 2: Anti-Aurora A (phospho-Thr288) Antibody (A50385)

Western blot analysis of extracts from HT29 cells, treated with Hydroxyurea or calf intestinal phosphatase (CIP), using Aurora A (phospho-Thr288) Antibody #11519.

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