Anti-ATR (phospho Ser428) Antibody (A91791)

Name: Anti-ATR (phospho Ser428) Antibody
Brand: Antibodies.com
SKU: A91791
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to ATR (phospho Ser428) for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-ATR (phospho Ser428) Antibody

Description

Rabbit polyclonal antibody to ATR (phospho Ser428).

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S428 of human ATR (NP_001175.2).

Sequence

GISPK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

300 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Ataxia telangiectasia and Rad3 related, Ataxia telangiectasia and Rad3-related protein, ATR_HUMAN, FCTCS, FRAP Related Protein 1, FRAP-related protein 1, FRP1, MEC1, MEC1 mitosis entry checkpoint 1 homolog, Protein kinase ATR, RAC3, Rad3 related protein, SCKL, SCKL1, Serine/threonine protein kinase ATR, Serine/threonine-protein kinase ATR

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-ATR (phospho Ser428) Antibody (A91791)

Western blot analysis of 293T, using Anti-ATR (phospho Ser428) Antibody (A91791) at 1:400 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-ATR (phospho Ser428) Antibody (A91791)

Western blot analysis of NIH/3T3, using Anti-ATR (phospho Ser428) Antibody (A91791) at 1:400 dilution. NIH/3T3 cells were treated by Nocodazole (50 ng/ml) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-ATR (phospho Ser428) Antibody (A91791)

Immunohistochemistry analysis of paraffin-embedded human testis using Anti-ATR (phospho Ser428) Antibody (A91791) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-ATR (phospho Ser428) Antibody (A91791)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-ATR (phospho Ser428) Antibody (A91791) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.