Anti-ATG9B Antibody (A304906)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-ATG9B Antibody (A304906), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit IgG: Alexa Fluor 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasmic Vesicle, Autophagosome Membrane, Multi-Pass membrane Protein. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG9B Antibody (D) Composite.

Western blot analysis of rat brain cell lysates showing detection of 101 kDa ATG9B protein using Anti-ATG9B Antibody (A304906) at 1:1,000 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat brain cell lysates. Load: 15 µg. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: 101 kDa.