Anti-ATG9B Antibody (A10143)

Name: Anti-ATG9B Antibody
Brand: Antibodies.com
SKU: A10143
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to ATG9B for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-ATG9B Antibody

Description

Rabbit polyclonal antibody to ATG9B.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 715-924 of human ATG9B (NP_775952.4).

Sequence

HPLWRPPGHSSKFLGHLWGRVQQDAAAWGATSARGPSTPGVLSNCTSPLPEAFLANLFVHPLLPPRDLSPTAPCPAAATASLLASISRIAQDPSSVSPGGTGGQKLAQLPELASAEMSLHVIYLHQLHQQQQQQEPWGEAAASILSRPCSSPSQPPSPDEEKPSWSSDGSSPASSPRQQWGTQKARNLFPGGFQVTTDTQKEPDRASCTD

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

100 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

APG9 like 2, APG9-like 2, Apg912, APG9L2, Apgdc2, ATG9 autophagy related 9 homolog B (S. cerevisiae), ATG9B_HUMAN, Autophagy 9 like 2 protein, Autophagy related protein 9B, Autophagy-related protein 9B, Endothelial nitric oxide synthase antisense, eONE, Gm574, Nitric oxide synthase 3 antisense, Nitric oxide synthase 3 overlapping antisense gene protein, Nitric oxide synthase 3-overlapping antisense gene protein, NOS3AS, Protein sONE, SONE

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-ATG9B Antibody (A10143)

Western blot analysis of extracts of HUH-7 cells, using Anti-ATG9B Antibody (A10143) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-ATG9B Antibody (A10143)

Western blot analysis of extracts of various cell lines, using Anti-ATG9B Antibody (A10143) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunofluorescence - Anti-ATG9B Antibody (A10143)

Immunofluorescence analysis of human placenta cells using Anti-ATG9B Antibody (A10143) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

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