Anti-ATG7 Antibody [ARC0083] (A306787)

Name: Anti-ATG7 Antibody [ARC0083]
Brand: Antibodies.com
SKU: A306787
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0083] antibody to ATG7 for WB, IHC and IP.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Knockout Validated Antibody Peroxisome Marker

Specifications

Name

Anti-ATG7 Antibody [ARC0083]

Description

Rabbit monoclonal [ARC0083] antibody to ATG7.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Atg7(Apg7) (O95352).

Sequence

MAAATGDPGLSKLQFAPFSSALDVGFWHELTQKKLNEYRLDEAPKDIKGYYYNGDSAGLPARLTLEFSAFDMSAPTPARCCPAIGTLYNTNTLESFKTAD

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0083

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

77 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

1810013K23Rik, Apg 7, Apg7, APG7 autophagy 7 like, APG7 autophagy 7-like (S. cerevisiae), APG7 like, APG7, S. cerevisiae, homolog of, APG7-like, APG7L, ATG 7, ATG12-activating enzyme E1 ATG7, ATG7 autophagy related 7 homolog, ATG7 autophagy related 7 homolog (S. cerevisiae), Atg7l, ATG7_HUMAN, Autophagy 7, S. cerevisiae, homolog of, Autophagy related protein 7, Autophagy-related 7 (yeast), Autophagy-related protein 7, DKFZp434N0735, GSA 7, GSA7, hAGP7, Ubiquitin activating enzyme E1 like protein, Ubiquitin-activating enzyme E1-like protein, Ubiquitin-like modifier-activating enzyme ATG7

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-ATG7 Antibody [ARC0083] (A306787)

Western blot analysis of extracts of various cell lines, using Anti-ATG7 Antibody [ARC0083] (A306787) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-ATG7 Antibody [ARC0083] (A306787)

Western blot analysis of extracts from wild type(WT) and HNRNPF knockout (KD) Human cells, using Anti-ATG7 Antibody [ARC0083] (A306787) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: ARC0083.

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Figure 3: Immunohistochemistry - Anti-ATG7 Antibody [ARC0083] (A306787)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-ATG7 Antibody [ARC0083] (A306787) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-ATG7 Antibody [ARC0083] (A306787)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-ATG7 Antibody [ARC0083] (A306787) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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