Anti-ATG4C Antibody (A304856)

Name: Anti-ATG4C Antibody
Brand: Antibodies.com
SKU: A304856
Price: 500.0 USD
Availability: InStock

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$500

Rabbit polyclonal antibody to ATG4C for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-ATG4C Antibody

Description

Rabbit polyclonal antibody to ATG4C.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivity

Human

Immunogen

Synthetic peptide from the C-terminal of Human ATG4C (aa. 296-306).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Peptide affinity purification.

Concentration

1 mg/ml

Molecular Weight

~50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

APG4 autophagy 4 homolog C, APG4 autophagy 4 homolog C (S. cerevisiae), APG4 C, APG4-C, APG4C, ATG 4C, ATG4 autophagy related 4 homolog C, ATG4 autophagy related 4 homolog C (S. cerevisiae), ATG4C_HUMAN, AUT (S. cerevisiae) like 1, cysteine endopeptidase, AUT like 1, cysteine endopeptidase, AUT like 1, cysteine endopeptidase (S. cerevisiae), AUT like 3 cysteine endopeptidase, AUT-like 3 cysteine endopeptidase, AUTL1, AUTL3, Autophagin 3, Autophagin-3, Autophagy related 4C cysteine peptidase, Autophagy related cysteine endopeptidase 3, Autophagy related protein 4 homolog C, Autophagy-related cysteine endopeptidase 3, Autophagy-related protein 4 homolog C, Cysteine protease ATG4C, EC 3.4.22, FLJ14867, OTTHUMP00000010715

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-ATG4C Antibody (A304856)

Western blot analysis of human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of 53 kDa ATG4C protein using Anti-ATG4C Antibody (A304856) at 1:1,000 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder (MW). Lane 2: human HEK293T cell lysates. Load: 15 µg. Block: 2% GE Ashermsham in 1X TBST. The secondary antibody used was Goat Anti-Rabbit HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 minutes in room temperature. Predicted/Observed Size: 53 kDa.

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Figure 2: Immunofluorescence - Anti-ATG4C Antibody (A304856)

Immunocytochemistry/Immunofluorescence analysis of human colon carcinoma cell line (RKO), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-ATG4C Antibody (A304856), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasm. Magnification: 60X.(A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG4C Antibody. (D) Composite.

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