Anti-ATG3 Antibody [ARC0073] (A307672)

Name: Anti-ATG3 Antibody [ARC0073]
Brand: Antibodies.com
SKU: A307672
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0073] antibody to ATG3 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-ATG3 Antibody [ARC0073]

Description

Rabbit monoclonal [ARC0073] antibody to ATG3.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Apg3 (Atg3) (Q9NT62).

Sequence

MQNVINTVKGKALEVAEYLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWQWATGEELKVKAYLPTGKQFLVTKNVPCYKRCKQMEYSDELEAIIEE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0073

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

35 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

2610016C12Rik, Apg 3, Apg3, APG3 autophagy 3 like, APG3 like, APG3, S. cerevisiae, homolog of, APG3-like, APG3L, Apg3p, ATG 3, ATG3 autophagy related 3 homolog, ATG3 autophagy related 3 homolog (S. cerevisiae), ATG3_HUMAN, Autophagy 3, S. cerevisiae, homolog of, Autophagy Apg3p/Aut1p like, autophagy related 3, Autophagy related protein 3, Autophagy-related protein 3, DKFZp564M1178, FLJ22125, hApg3, MGC15201, OTTHUMP00000214547, OTTHUMP00000214548, PC3 96, Protein PC3-96, Ubiquitin-like-conjugating enzyme ATG3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-ATG3 Antibody [ARC0073] (A307672)

Western blot analysis of extracts of various cell lines, using Anti-ATG3 Antibody [ARC0073] (A307672) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 40s.

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Figure 2: Immunohistochemistry - Anti-ATG3 Antibody [ARC0073] (A307672)

Immunohistochemistry analysis of paraffin-embedded rat liver using Anti-ATG3 Antibody [ARC0073] (A307672) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-ATG3 Antibody [ARC0073] (A307672)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-ATG3 Antibody [ARC0073] (A307672) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-ATG3 Antibody [ARC0073] (A307672)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-ATG3 Antibody [ARC0073] (A307672) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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