Anti-ATG3 Antibody (A304845)

Name: Anti-ATG3 Antibody
Brand: Antibodies.com
SKU: A304845
Price: 500.0 USD
Availability: InStock

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$500

Rabbit polyclonal antibody to ATG3 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-ATG3 Antibody

Description

Rabbit polyclonal antibody to ATG3.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivity

Human

Immunogen

Synthetic peptide from the mid-protein of Human ATG3.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Peptide affinity purification.

Concentration

1 mg/ml

Molecular Weight

~35 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

2610016C12Rik, Apg 3, Apg3, APG3 autophagy 3 like, APG3 like, APG3, S. cerevisiae, homolog of, APG3-like, APG3L, Apg3p, ATG 3, ATG3 autophagy related 3 homolog, ATG3 autophagy related 3 homolog (S. cerevisiae), ATG3_HUMAN, Autophagy 3, S. cerevisiae, homolog of, Autophagy Apg3p/Aut1p like, autophagy related 3, Autophagy related protein 3, Autophagy-related protein 3, DKFZp564M1178, FLJ22125, hApg3, MGC15201, OTTHUMP00000214547, OTTHUMP00000214548, PC3 96, Protein PC3-96, Ubiquitin-like-conjugating enzyme ATG3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-ATG3 Antibody (A304845)

Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of ~35.9 kDa ATG3 protein using Anti-ATG3 Antibody (A304845) at 1:1,000 for 60 minutes at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~35.9 kDa.

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Figure 2: Immunofluorescence - Anti-ATG3 Antibody (A304845)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-ATG3 Antibody (A304845), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasm. Magnification: 60X.(A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG3 Antibody. (D) Composite.

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