Anti-ATF6 Antibody (A85123)

Name: Anti-ATF6 Antibody
Brand: Antibodies.com
SKU: A85123
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to ATF6 for ELISA, WB, IF and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-ATF6 Antibody

Description

Goat polyclonal antibody to ATF6.

Applications

ELISA, WB, IF, Flow Cytometry

Dilutions

ELISA: 1:128,000, WB: 0.3-2µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region, amino acids 437-449, of human ATF6.

Sequence

C-NSYRYDHSVSNDK

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

75-80 kDa

Predicted MW

74.6kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Activating transcription factor 6, Activating transcription factor 6 alpha, ATF 6, ATF6 alpha, ATF6-alpha, ATF6A, ATF6A_HUMAN, cAMP dependent transcription factor ATF 6 alpha, cAMP-dependent transcription factor ATF-6 alpha, Cyclic AMP dependent transcription factor ATF 6 alpha, DKFZp686P2194, ESTM49, FLJ21663, Processed cyclic AMP dependent transcription factor ATF 6 alpha, Processed cyclic AMP-dependent transcription factor ATF-6 alpha

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-ATF6 Antibody (A85123)

ATF6 expression in HeLa (A), MCF7 (B), HeLa nuclear (C), and Jurkat nuclear (D) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-ATF6 Antibody (A85123) at 1µg/ml and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-ATF6 Antibody (A85123)

ATF6 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ATF6 Antibody (A85123) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Immunofluorescence - Anti-ATF6 Antibody (A85123)

ATF6 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ATF6 Antibody (A85123) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Flow Cytometry - Anti-ATF6 Antibody (A85123)

ATF6 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-ATF6 Antibody (A85123) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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